Plant Biology: Photosynthesis and Reproduction Notes
Exam notes on plant biology: photosynthesis, its light and dark reactions, C3, C4 and CAM plants, flower parts, pollination, double fertilisation and seeds.
By GK24 Editorial Team· Published · 5 min read

Plants are the only large group of organisms that can build their own food from inorganic raw material, which makes them autotrophs and places them at the base of almost every food chain on land. Two processes decide how a plant lives and how it continues: photosynthesis, by which it captures sunlight and stores it as chemical energy, and reproduction, by which it passes that ability to the next generation. Both are heavy scoring areas in general science papers because the facts are fixed and can be asked directly: the site of a reaction, the metal in a pigment, the first product of a pathway, the part of a flower that becomes a fruit.
Photosynthesis: the overall reaction
Photosynthesis is the process by which green plants use carbon dioxide and water, in the presence of sunlight and chlorophyll, to make carbohydrate and release oxygen. In its simplest written form, six molecules of carbon dioxide and six of water yield one molecule of glucose and six of oxygen. The raw materials are drawn from two directions: carbon dioxide enters through the stomata on the leaf, and water arrives from the roots through the xylem. The energy converted is light energy turning into chemical energy stored in the bonds of the carbohydrate. The oxygen given off does not come from the carbon dioxide at all; it comes from the splitting of water, a point settled by experiments using heavy oxygen as a tracer.
Chloroplast, pigments and the two reactions
Photosynthesis takes place in the chloroplast. Its stacked membranes, the grana made of thylakoids, hold the pigments and carry out the light reaction, while the fluid-filled stroma around them carries out the dark reaction. The chief pigment is chlorophyll, which has magnesium at the centre of its molecule; iron, by contrast, sits at the centre of haemoglobin, and the two are regularly swapped in questions. Chlorophyll absorbs blue and red light strongly and reflects green, which is why leaves look green, and the rate of photosynthesis is highest in red light. Accessory pigments such as carotenoids and xanthophylls give the orange and yellow colours seen in autumn leaves and in carrot roots.
| Feature | Light reaction | Dark reaction |
|---|---|---|
| Other name | Hill reaction, photophosphorylation | Calvin cycle, biosynthetic phase |
| Site | Grana or thylakoid membranes | Stroma of the chloroplast |
| Light needed | Yes | No, but it uses the products of the light reaction |
| Main events | Splitting of water, release of oxygen | Fixation of carbon dioxide into carbohydrate |
| Products | ATP, NADPH and oxygen | Glucose |
C3, C4 and CAM plants
The dark reaction fixes carbon dioxide with the help of the enzyme RuBisCO, held to be the most abundant enzyme on earth. In most plants, called C3 plants, carbon dioxide joins a five-carbon sugar and the first stable product is the three-carbon compound 3-phosphoglyceric acid, which gives the group its name. In C4 plants such as maize, sugarcane and sorghum, carbon dioxide is first fixed by a different enzyme into the four-carbon compound oxaloacetic acid, a route named the Hatch and Slack pathway; these plants have a special leaf structure called Kranz anatomy, waste almost nothing to photorespiration and do well in hot, bright conditions. CAM plants such as pineapple and prickly pear open their stomata at night, store the carbon dioxide as an acid and use it by day, which saves water in dry habitats. Blackman's law of limiting factors explains why the rate stops rising when any one factor, usually carbon dioxide or light, runs short.
Reproduction without seeds
Many plants multiply without gametes, so the new plant is genetically identical to its parent and flowers sooner. Natural vegetative propagation works through modified stems and roots: the potato tuber sprouts from its eyes, ginger and turmeric spread by rhizomes, onion and garlic by bulbs, grass by runners and banana by suckers; bryophyllum is the classic example of a plant that grows new shoots from buds on the notches of its leaf. Gardeners use artificial methods such as cutting, layering, grafting and budding, and tissue culture raises thousands of identical disease-free plants from a few cells in the laboratory.
The flower and double fertilisation
In flowering plants the flower is the reproductive organ. From the outside inwards it has four whorls: the calyx of sepals, the corolla of petals, the androecium of stamens and the gynoecium of carpels. A stamen, the male part, is made of a filament and an anther, and the anther produces pollen grains. A carpel, the female part, has a stigma that receives pollen, a style and an ovary that holds the ovules. Pollination is the transfer of pollen from anther to stigma; it is self-pollination within the same flower or plant and cross-pollination between two plants, carried by wind, water, insects, birds or bats. After pollination the pollen grain grows a tube to the ovule and releases two male gametes. One fuses with the egg to make a diploid zygote and the other fuses with the two polar nuclei to make a triploid primary endosperm nucleus. This event, two fusions in one ovule, is double fertilisation, and it is found only in angiosperms.
Fruit, seed and hormones
Once fertilisation is over the ovary ripens into the fruit and each ovule becomes a seed, while the ovary wall becomes the pericarp. A fruit formed from the ovary alone is a true fruit; apple and pear, in which the fleshy part comes from the thalamus, are called false fruits. A fruit that forms without fertilisation, as in the banana, is a parthenocarpic fruit. A seed carries the embryo with its plumule and radicle, the stored food in cotyledons or endosperm, and a protective seed coat; maize with one cotyledon is a monocot and gram with two is a dicot. In germination, the cotyledons come above the soil in epigeal types such as castor and bean, but stay below in hypogeal types such as pea and gram. Growth is steered by hormones: auxin causes bending towards light and apical dominance, gibberellin lengthens stems, cytokinin promotes cell division, abscisic acid is the stress hormone that closes stomata, and ethylene, a gas, ripens fruit.
Exam Point of View
Most questions are single-fact recall. Expect the site of a reaction (grana for light, stroma for dark), the metal in chlorophyll, the source of released oxygen, and the first product of the C3 and C4 pathways, where oxaloacetic acid and 3-phosphoglyceric acid are deliberately exchanged. A second group asks you to pair a plant with its modified organ: potato with stem, sweet potato with root, ginger with rhizome, onion with bulb. A third group tests flower structure and the vocabulary around it, and the favourite trap is to ask which part becomes the fruit, where candidates answer ovule instead of ovary, or to ask the ploidy of the endosperm, which is triploid. Double fertilisation being unique to angiosperms, the example plants of C4 and CAM groups, epigeal against hypogeal germination, and one hormone to one function are the other reliable sources of questions.
Important Facts
| Site of photosynthesis | Chloroplast; grana for the light reaction, stroma for the dark reaction |
|---|---|
| Metal in chlorophyll | Magnesium |
| Source of released oxygen | Water, by photolysis in the light reaction |
| Light reaction products | ATP, NADPH and oxygen |
| Carbon dioxide fixing enzyme | RuBisCO, the most abundant enzyme on earth |
| First product in C3 plants | 3-phosphoglyceric acid, a three-carbon compound |
| First product in C4 plants | Oxaloacetic acid, a four-carbon compound |
| C4 pathway | Hatch and Slack pathway, with Kranz anatomy in the leaf |
| C4 examples | Maize, sugarcane, sorghum and amaranthus |
| CAM examples | Pineapple and prickly pear (Opuntia); stomata open at night |
| Male part of flower | Stamen, made of filament and anther |
| Female part of flower | Carpel, made of stigma, style and ovary |
| Double fertilisation | Syngamy plus triple fusion; found only in angiosperms |
| Ploidy of endosperm | Triploid (3n) |
| After fertilisation | Ovary becomes fruit, ovule becomes seed, ovary wall becomes pericarp |
| False fruits | Apple and pear, in which the thalamus forms the fleshy part |
| Parthenocarpic fruit | Banana, formed without fertilisation |
| Fruit ripening hormone | Ethylene, the only gaseous plant hormone |
Practice MCQs on this topic
Photosynthesis in plants converts light energy to ______ energy.
- A.thermal
- B.chemical
- C.kinetic
- D.physical
Show answer
Correct answer: B. chemical
Explanation
The correct answer is B, chemical. In photosynthesis a green plant absorbs sunlight through chlorophyll and uses that energy to join carbon dioxide and water into carbohydrate. The energy of the light does not disappear; it is locked into the chemical bonds of the glucose molecule, which is why the leaf is described as converting light energy into chemical energy. That stored energy is later released by respiration in the plant, or in the animal that eats it. A is wrong because although leaves do warm slightly in sunlight, heat is an incidental loss and not the product of photosynthesis. C is wrong because kinetic energy is the energy of a moving body and nothing in the leaf is set in motion by the process. D is wrong because physical energy is not a recognised category of energy at all; it is a vague term included to mislead a hurried reader.
Which metal is present at the centre of the chlorophyll molecule?
- A.Iron
- B.Magnesium
- C.Calcium
- D.Zinc
Show answer
Correct answer: B. Magnesium
Explanation
The correct answer is B, magnesium. Chlorophyll is built on a ring structure with a single magnesium atom held at its centre, and because magnesium is needed to make the pigment, a plant short of magnesium develops chlorosis, the yellowing of leaves between the veins. A is wrong, and is the commonest error, because iron sits at the centre of the haem group of haemoglobin, the respiratory pigment of blood; iron is still needed by plants, but for enzymes and for chlorophyll synthesis rather than as part of the molecule. C is wrong because calcium in plants is used mainly in the cell wall as calcium pectate and in cell signalling. D is wrong because zinc is a micronutrient needed in trace amounts by several enzymes. A useful pair to memorise together is magnesium in chlorophyll, iron in haemoglobin, cobalt in vitamin B12.
The oxygen released during photosynthesis comes from
- A.Carbon dioxide
- B.Water
- C.Glucose
- D.Chlorophyll
Show answer
Correct answer: B. Water
Explanation
The correct answer is B, water. In the light reaction, water absorbed by the roots is split in the presence of light and chlorophyll, a step called photolysis of water or the Hill reaction. The hydrogen freed is carried away to reduce NADP, and the oxygen atoms combine and escape as oxygen gas through the stomata. Experiments that fed plants water labelled with heavy oxygen found the label in the gas released, which settled the matter. A is wrong because the carbon and oxygen of carbon dioxide are built into the carbohydrate rather than released, though the name of the gas tempts candidates. C is wrong because glucose is a product of the process, not a raw material being broken down. D is wrong because chlorophyll only absorbs light and passes on the energy; it is a catalyst-like pigment that is not consumed and contributes no oxygen.
The dark reaction of photosynthesis takes place in which part of the chloroplast?
- A.Grana
- B.Stroma
- C.Outer membrane
- D.Thylakoid lumen
Show answer
Correct answer: B. Stroma
Explanation
The correct answer is B, the stroma, the fluid-filled ground substance of the chloroplast that surrounds the membrane stacks and holds the enzymes of the Calvin cycle, including RuBisCO. Here the ATP and NADPH made in the light reaction are spent on fixing carbon dioxide into carbohydrate. A is wrong because the grana are the stacks of thylakoid membranes that carry the pigments and run the light reaction; placing the dark reaction there is the classic reversal in this question. C is wrong because the outer membrane of the chloroplast is only a boundary and has no role in either reaction. D is wrong because the thylakoid lumen is the space inside those membranes where protons accumulate during the light reaction to drive ATP synthesis, again part of the light phase. Remember the pair: light reaction in grana, dark reaction in stroma.
The first stable product of carbon dioxide fixation in C4 plants is
- A.3-phosphoglyceric acid
- B.Oxaloacetic acid
- C.Pyruvic acid
- D.Ribulose bisphosphate
Show answer
Correct answer: B. Oxaloacetic acid
Explanation
The correct answer is B, oxaloacetic acid, a four-carbon acid, which is exactly why the group is called C4. The gas is first fixed in the mesophyll cells by the enzyme PEP carboxylase on to a three-carbon acceptor, and the pathway is named after Hatch and Slack. Maize, sugarcane, sorghum and amaranthus follow it and show the special leaf anatomy called Kranz anatomy. A is wrong because 3-phosphoglyceric acid is the three-carbon first product of C3 plants such as wheat and rice; the two are swapped in almost every paper that asks this. C is wrong because pyruvic acid is a product of glycolysis in respiration and appears in the C4 cycle only later, when the acid is regenerated. D is wrong because ribulose bisphosphate is the five-carbon acceptor molecule in the Calvin cycle, not a product of fixation at all.
Which of the following parts of a flower forms part of stamen?
- A.Style
- B.Anther
- C.Stigma
- D.Sepal
Show answer
Correct answer: B. Anther
Explanation
The correct answer is B, the anther. A stamen is the male reproductive unit of a flower and has just two parts: a stalk called the filament and a swollen head called the anther, in which pollen grains are produced. All the stamens of a flower together make up the whorl called the androecium. A is wrong because the style is the slender tube of the carpel that connects the stigma to the ovary, so it belongs to the female whorl. C is wrong for the same reason: the stigma is the sticky tip of the carpel that receives pollen, and candidates who remember only that pollen touches the stigma often pick it by mistake. D is wrong because a sepal is a member of the calyx, the outermost green whorl that protects the bud; it has no reproductive role. The pair to fix in memory is filament and anther for the stamen, stigma, style and ovary for the carpel.
Double fertilisation is a characteristic feature of which group of plants?
- A.Bryophytes
- B.Pteridophytes
- C.Gymnosperms
- D.Angiosperms
Show answer
Correct answer: D. Angiosperms
Explanation
The correct answer is D, angiosperms, the flowering plants. Their pollen tube carries two male gametes into the ovule; one fuses with the egg to form the diploid zygote, which is syngamy, and the other fuses with the two polar nuclei to form the triploid primary endosperm nucleus, which is triple fusion. Two fusions in a single ovule make the event double fertilisation, and it is found nowhere else in the plant kingdom. A is wrong because bryophytes such as mosses have no pollen tube at all and need water for their motile male gametes to swim to the egg. B is wrong because pteridophytes such as ferns also release swimming gametes and bear no seeds. C is wrong because gymnosperms do form pollen tubes and seeds, but only one gamete fuses with the egg and their nutritive tissue is haploid female tissue, not a triploid endosperm.
After fertilisation, which part of the flower develops into the fruit?
- A.Ovule
- B.Ovary
- C.Style
- D.Petal
Show answer
Correct answer: B. Ovary
Explanation
The correct answer is B, the ovary. Once fertilisation is complete, the ovary enlarges and ripens into the fruit, and its wall becomes the pericarp that may be fleshy, as in a mango, or dry, as in a pea pod. A is wrong, and is the single commonest error in this question, because each ovule inside the ovary becomes a seed, not the fruit; the number of seeds in a fruit therefore tells you how many ovules the ovary held. C is wrong because the style, like the stigma, usually withers and falls away after pollination, having served only to carry the pollen tube down to the ovule. D is wrong because petals are display organs that attract pollinators and drop off soon after pollination. Note also that a fruit formed from the ovary alone is a true fruit, while apple and pear, whose flesh comes from the thalamus, are false fruits.
The potato is a modified
- A.Root
- B.Stem
- C.Leaf
- D.Flower
Show answer
Correct answer: B. Stem
Explanation
The correct answer is B, a stem. The potato is an underground stem tuber, and the proof is on its skin: the hollows called eyes are axillary buds with tiny scale leaves, and buds of this kind are never found on a true root. That is why a cut piece of potato with an eye grows into a whole new plant. A is wrong because the familiar modified roots are the sweet potato, carrot, radish, turnip and beetroot, none of which carries buds. C is wrong because modified leaves give us the fleshy scales of an onion bulb, the tendrils of a pea and the spines of a cactus, but not the potato. D is wrong because the potato is not a reproductive structure in the floral sense; the potato plant does bear flowers separately. Group the modified stems for revision: potato tuber, ginger and turmeric rhizomes, onion and garlic bulbs, colocasia corm.
Which plant hormone is mainly responsible for the ripening of fruits?
- A.Auxin
- B.Gibberellin
- C.Ethylene
- D.Cytokinin
Show answer
Correct answer: C. Ethylene
Explanation
The correct answer is C, ethylene, the only plant hormone that is a gas. It hastens the ripening of fruit, encourages leaves and flowers to drop and is used commercially in the form of ethephon to ripen mangoes, bananas and tomatoes evenly; a ripe fruit kept with unripe ones ripens them because it gives off ethylene. A is wrong because auxin is the hormone of cell elongation, bending towards light and apical dominance, and in high doses it works as a weedkiller. B is wrong because gibberellin lengthens stems, breaks seed dormancy and causes bolting in rosette plants; it is associated with growth in height rather than ripening. D is wrong because cytokinin promotes cell division, delays the ageing of leaves and helps shoots form in tissue culture. The hormone to pair with stress is abscisic acid, which closes the stomata and enforces dormancy.
Frequently Asked Questions
Where does the oxygen released in photosynthesis come from?
It comes from water, not from carbon dioxide. During the light reaction, water is split in the presence of light and chlorophyll, a step called photolysis or the Hill reaction, and the oxygen atoms freed from water escape as oxygen gas. Experiments using heavy oxygen as a tracer confirmed this, and it is a favourite one-line question.
What is the difference between the light reaction and the dark reaction?
The light reaction needs light, happens on the grana or thylakoid membranes, splits water and produces ATP, NADPH and oxygen. The dark reaction, or Calvin cycle, happens in the stroma, needs no light of its own and uses the ATP and NADPH made earlier to fix carbon dioxide into carbohydrate. The dark reaction can occur in daylight; its name only means light is not directly required.
Why are C4 plants more efficient than C3 plants in hot climates?
C4 plants fix carbon dioxide twice. The first fixation, by an enzyme with a strong appetite for carbon dioxide, concentrates the gas in bundle sheath cells, so RuBisCO works in a carbon dioxide rich pocket and almost no photorespiration occurs. They can also keep their stomata partly closed and still photosynthesise, which saves water under strong sun.
What is double fertilisation and why is it special?
The pollen tube delivers two male gametes into the ovule. One fuses with the egg to form a diploid zygote, called syngamy, and the other fuses with the two polar nuclei to form a triploid primary endosperm nucleus, called triple fusion. Two fusions in one ovule make it double fertilisation, and it occurs only in angiosperms, the flowering plants.
Which part of the flower becomes the fruit and which becomes the seed?
The ovary ripens into the fruit and its wall becomes the pericarp, while each ovule inside the ovary becomes a seed. Candidates often reverse the two. If the fleshy part comes from a region other than the ovary, as the thalamus does in apple and pear, the result is called a false fruit.
Is the potato a root or a stem?
The potato is a modified underground stem known as a tuber. The proof is on its surface: the eyes are buds with scale leaves, and a true root has no buds. Sweet potato and carrot, on the other hand, are modified roots. Ginger and turmeric are rhizomes and onion is a bulb, both again modified stems.
Sources
- Biology (Class XI), Chapter: Photosynthesis in Higher Plants — NCERT
- Biology (Class XII), Chapter: Sexual Reproduction in Flowering Plants — NCERT
- Science (Class X), Chapter: Life Processes — NCERT
- Biology (Class XI), Chapter: Morphology of Flowering Plants — NCERT





