Food Preservation, Adulteration and Food Safety Notes
Exam notes on food preservation methods, Class I and II preservatives, common adulterants with their tests and diseases, FSSAI, Codex and food safety marks.
By GK24 Editorial Team· Published · 4 min read

Food begins to spoil the moment it is harvested or slaughtered, and almost every technique of food preservation is an attempt to stop the three agents of that spoilage: micro-organisms, the food's own enzymes, and oxygen. Food safety law adds a second layer by fixing what may be added to food and what may not, and by punishing adulteration. The subject is a steady favourite in General Science and General Awareness papers, and it returns to the news whenever the food regulator issues fresh standards, such as the draft rules on pesticide residue limits in tea and on added sugars in infant foods. The lasting facts below are what examiners actually ask.
Why food spoils
Bacteria, yeasts and moulds need moisture, warmth, a near-neutral pH and oxygen to multiply. Remove any one of these and growth slows or stops. Enzymes inside the food continue to act even after harvest, which is why a cut apple browns and why peas lose sweetness. Fats turn rancid by oxidation. Preservation therefore works by one of four strategies: killing the micro-organisms, removing their water, making the surroundings hostile to them, or slowing every chemical reaction by cooling. Using two or three mild methods together instead of one harsh one is called hurdle technology, and it is how most packaged food is kept safe today.
Methods of preservation
Low temperature does not kill microbes; it only slows them. Refrigeration at about 0 to 5 degrees Celsius slows respiration and microbial growth, which is why fruit kept in a cold chamber has a longer storage life. Freezing at about minus eighteen degrees Celsius halts growth altogether. Heat destroys microbes: pasteurisation of milk uses either the low-temperature long-time method at about 63 degrees Celsius for thirty minutes or the high-temperature short-time method at about 72 degrees Celsius for fifteen seconds, followed by rapid chilling. Ultra-high temperature treatment uses roughly 135 to 150 degrees Celsius for a few seconds and gives milk that keeps unopened for months. Canning, invented by Nicolas Appert, seals food in an airtight container and then heats it; Louis Pasteur later explained why such heating works.
Drying and dehydration remove the water microbes need. Freeze-drying, or lyophilisation, freezes the food and then removes the ice directly as vapour under vacuum by sublimation, which preserves flavour and is used for instant coffee and vaccines. Osmotic methods bind the remaining water: heavy salting cures fish and meat, high sugar concentration preserves jam and squash, and acid in vinegar or in oil pickles lowers the pH below what spoilage organisms tolerate. Fermentation lets harmless lactic acid bacteria acidify the food themselves, as in curd, idli batter and pickles.
Chemical preservatives are divided by Indian food standards into two classes. Class I preservatives are natural and may be used without restriction: common salt, sugar, dextrose, spices, vinegar, honey and edible vegetable oils. Class II preservatives are chemical and are permitted only in listed foods and listed quantities: benzoic acid and sodium benzoate, sulphur dioxide and sulphites, sorbic acid and sorbates, nitrites and nitrates for cured meat, and propionates against mould in bread. Antioxidants such as ascorbic acid and tocopherols are added against rancidity rather than against microbes. Irradiation exposes food to gamma rays from a cobalt-60 source to kill insects, delay sprouting in onions and potatoes and destroy pathogens; it does not make the food radioactive, and irradiated food carries the international Radura symbol. Vacuum packing and modified atmosphere packaging replace oxygen with carbon dioxide or nitrogen.
Common adulterants and simple tests
| Food | Adulterant | Effect or test |
|---|---|---|
| Mustard oil | Argemone (satyanashi) seed oil | Causes epidemic dropsy; nitric acid test |
| Arhar or besan | Khesari dal (Lathyrus sativus) | Causes lathyrism, a paralysis of the lower limbs |
| Turmeric and pulses | Metanil yellow, lead chromate | Non-permitted coal-tar dye; hydrochloric acid test |
| Milk and khoa | Starch, water, detergent, urea | Iodine solution turns starch blue-black |
| Chilli powder | Brick powder, sawdust | Sediment settles or floats in a glass of water |
| Black pepper | Papaya seeds | Lighter papaya seeds float in water |
| Ghee | Vanaspati, animal fat | Hydrochloric acid and sugar test turns crimson |
| Coffee powder | Chicory | Chicory sinks with a colour trail in water |
Calcium carbide, once used to ripen mangoes artificially, is banned in India because it releases acetylene and carries traces of arsenic and phosphorus; controlled ripening with ethylene gas is the permitted alternative. Formalin in fish, oxytocin injected into milch animals and industrial dyes in sweets are other prohibited practices.
Food safety law in India
The Food Safety and Standards Act, 2006 replaced a group of older laws, chief among them the Prevention of Food Adulteration Act, 1954, and created a single regulator, the Food Safety and Standards Authority of India, which works under the Ministry of Health and Family Welfare and has its headquarters at New Delhi. Every food business must hold an FSSAI licence or registration, and the number printed on the pack lets a buyer trace the manufacturer. Internationally, food standards are set by the Codex Alimentarius Commission, created jointly by the Food and Agriculture Organization and the World Health Organization. World Food Safety Day is observed on 7 June and National Consumer Day in India on 24 December. Fortified food carries the plus-F logo, agricultural produce graded under the Agricultural Produce (Grading and Marking) Act carries the AGMARK seal, and processed goods under compulsory certification carry the ISI mark of the Bureau of Indian Standards.
Exam Point of View
Examiners take three kinds of question from this topic. First, adulterant and disease pairs: argemone oil with epidemic dropsy and khesari dal with lathyrism are asked almost every year, and the trap is to swap the two. Second, the technical name of a method: lyophilisation for freeze-drying, sublimation as the change of state involved, cobalt-60 as the gamma source, and the exact pasteurisation figures, where 63 degrees for 30 minutes and 72 degrees for 15 seconds are offered as alternatives. Third, the institutions and years: the Act of 2006, FSSAI under the Health Ministry, the 1954 Act it replaced, and Codex under the FAO and the WHO, where the usual distractor is UNESCO or the WTO. Class I versus Class II preservatives is the standard HARD question; remember that anything from the kitchen is Class I.
Important Facts
| Governing Act | Food Safety and Standards Act, 2006 |
|---|---|
| Act replaced | Prevention of Food Adulteration Act, 1954 |
| FSSAI ministry | Ministry of Health and Family Welfare; headquarters New Delhi |
| HTST pasteurisation | About 72 degrees Celsius for 15 seconds, then rapid chilling |
| LTLT pasteurisation | About 63 degrees Celsius for 30 minutes |
| Freeze-drying | Lyophilisation; ice removed as vapour by sublimation under vacuum |
| Irradiation source | Gamma rays from cobalt-60; mark is the Radura symbol |
| Epidemic dropsy | Argemone (satyanashi) oil in mustard oil |
| Lathyrism | Khesari dal, Lathyrus sativus |
| Starch test | Iodine solution gives a blue-black colour |
| Banned ripening agent | Calcium carbide; ethylene gas is permitted |
| International standards | Codex Alimentarius Commission of the FAO and the WHO |
| World Food Safety Day | 7 June |
| Quality marks | AGMARK for agricultural produce, ISI of BIS for processed goods, plus-F for fortified food |
Practice MCQs on this topic
Fruits stored in a cold chamber exhibit longer storage life because
- A.exposure to sunlight is prevented
- B.concentration of carbon dioxide in the environment is increased
- C.rate of respiration is decreased
- D.there is an increase in humidity
Show answer
A harvested fruit is still a living tissue that keeps respiring, using up its own sugars and acids and giving off heat, carbon dioxide and ethylene.
Read the full explanation →The adulteration of mustard oil with argemone oil causes which disease?
- A.Lathyrism
- B.Epidemic dropsy
- C.Fluorosis
- D.Minamata disease
Show answer
Argemone mexicana, the prickly poppy known in India as satyanashi, has seeds that look very like mustard seeds, so its oil is a classic adulterant of mustard oil.
Read the full explanation →Lathyrism in human beings is caused by the prolonged consumption of which of the following?
- A.Khesari dal
- B.Argemone seeds
- C.Metanil yellow
- D.Chicory
Show answer
Khesari dal, botanically Lathyrus sativus and also called grass pea, is a hardy pulse that survives drought and is therefore sometimes mixed with arhar dal or besan.
Read the full explanation →In the high-temperature short-time (HTST) method of pasteurisation, milk is heated to about:
- A.63 degrees Celsius for 30 minutes
- B.72 degrees Celsius for 15 seconds
- C.100 degrees Celsius for 10 minutes
- D.150 degrees Celsius for 2 seconds
Show answer
Pasteurisation is not sterilisation: it is a mild heating that kills disease-causing organisms such as Mycobacterium tuberculosis, Salmonella and Brucella and most spoilage bacteria, while leaving the taste and nutritive value of milk largely unchanged.
Read the full explanation →Lyophilisation, used to make instant coffee and to preserve vaccines, is the technical name for which process?
- A.Pasteurisation
- B.Freeze-drying
- C.Canning
- D.Irradiation
Show answer
In lyophilisation the material is first frozen and then placed under a strong vacuum, so that the ice changes directly into vapour without passing through the liquid state.
Read the full explanation →Which of the following is a Class I preservative under Indian food standards?
- A.Sodium benzoate
- B.Sulphur dioxide
- C.Common salt
- D.Potassium sorbate
Show answer
Indian food standards divide preservatives into two classes. Class I preservatives are the natural ones that cooks have always used, and no limit is placed on the quantity: common salt, sugar, dextrose, spices, vinegar or acetic acid, honey and edible vegetable oils.
Read the full explanation →Which substance, banned in India, was misused for the artificial ripening of fruits such as mangoes?
- A.Ethylene
- B.Calcium carbide
- C.Sodium chloride
- D.Ascorbic acid
Show answer
Lumps of calcium carbide hidden in a crate of mangoes react with the moisture in the air and release acetylene gas, which forces the fruit to colour quickly, though the pulp inside stays unripe and tasteless.
Read the full explanation →The Food Safety and Standards Authority of India (FSSAI) was set up under an Act of which year, and it works under which ministry?
- A.2006; Ministry of Health and Family Welfare
- B.1954; Ministry of Agriculture
- C.2011; Ministry of Consumer Affairs
- D.2008; Ministry of Commerce and Industry
Show answer
That Act consolidated several scattered food laws, the most important of them the Prevention of Food Adulteration Act of 1954, into a single statute and created one authority to lay down science-based standards for food, regulate its manufacture, storage, distribution, sale and import, and licence food businesses; its headquarters is…
Read the full explanation →The Codex Alimentarius Commission, which frames international food standards, was created jointly by:
- A.UNESCO and UNICEF
- B.FAO and WHO
- C.WTO and UNCTAD
- D.UNEP and FAO
Show answer
Codex Alimentarius is Latin for food code, and the Commission of that name draws up model standards, codes of practice, and limits for additives, contaminants and pesticide residues, which member countries use when writing their own food law and which serve as the reference in international food trade.
Read the full explanation →A few drops of iodine solution added to a sample of milk or khoa turn it blue-black. This indicates the presence of:
- A.Urea
- B.Starch
- C.Detergent
- D.Vanaspati
Show answer
Iodine forms a deep blue-black complex with the long coiled chains of amylose in starch, and this colour change is the standard classroom and field test for starch.
Read the full explanation →Food irradiation, used in India to delay the sprouting of onions and potatoes, commonly employs gamma rays from which source?
- A.Uranium-235
- B.Cobalt-60
- C.Carbon-14
- D.Iodine-131
Show answer
Cobalt-60 is an artificial radioisotope that emits penetrating gamma rays and has a half-life of about 5.27 years, which makes it a convenient and long-lasting source for irradiation plants.
Read the full explanation →Frequently Asked Questions
What is the difference between pasteurisation and sterilisation?
Pasteurisation is a mild heat treatment that kills disease-causing and most spoilage organisms while keeping the taste and nutrients of the food nearly unchanged, so pasteurised milk still has to be refrigerated and used within days. Sterilisation uses much higher temperatures for longer and destroys all micro-organisms including spores, so the food keeps for months unopened but its flavour and some vitamins are lost.
Does irradiated food become radioactive?
No. The food only passes through a beam of gamma rays from a cobalt-60 source and never touches the source, so it does not retain radioactivity, just as a chest X-ray does not make a patient radioactive. Irradiation kills insects and microbes and stops sprouting, and the pack carries the Radura symbol.
How can starch in milk be detected at home?
Boil a small sample of the milk, cool it, and add two or three drops of tincture of iodine or iodine solution. A blue-black colour shows that starch such as arrowroot, maida or rice starch has been added to thicken the milk and mask added water. Pure milk gives no such colour.
Which disease is caused by eating khesari dal for a long time?
Lathyrism, a spastic paralysis of the lower limbs caused by the neurotoxin in Lathyrus sativus. It appears when khesari dal forms a large part of the diet for several months, which is why its sale mixed with arhar dal or besan is an offence.
Which organisation lays down food standards in India and which one does so internationally?
In India the Food Safety and Standards Authority of India, created under the Act of 2006 and working under the Ministry of Health and Family Welfare, sets standards and licences food businesses. Internationally the Codex Alimentarius Commission, set up jointly by the FAO and the WHO, frames model standards and limits that countries follow in their own law and in food trade.
Sources
- The Food Safety and Standards Act, 2006 and the Regulations made under it — Food Safety and Standards Authority of India
- Biology, Class XII, Chapter on Microbes in Human Welfare — NCERT
- Codex Alimentarius: International Food Standards — FAO and WHO





