Ex-situ Conservation: Zoos, Botanical Gardens and Gene Banks
Exam notes on ex-situ conservation in India: the Central Zoo Authority, famous zoos and botanical gardens, gene banks, cryopreservation and captive breeding.
By GK24 Editorial Team· Published · 4 min read

Conservation of biodiversity is done in two ways. In-situ conservation protects a species where it lives, in national parks, wildlife sanctuaries, biosphere reserves, conservation and community reserves and sacred groves. Ex-situ conservation, the subject of this chapter, takes the species or its germplasm out of its habitat and keeps it alive in a zoo, a botanical garden, a gene bank or a breeding centre. The release of captive-bred Great Indian Bustards into the Desert National Park in Rajasthan in October 2026 is an example of how the two are meant to meet: animals bred ex-situ are returned to the wild.
The two methods compared
| In-situ conservation | Ex-situ conservation |
|---|---|
| Species protected in its natural habitat | Species or germplasm kept outside the habitat |
| Whole ecosystem is conserved with the species | Only the species or its genetic material is conserved |
| National parks, sanctuaries, biosphere reserves, conservation reserves | Zoos, botanical gardens, seed and gene banks, aquaria, safari parks |
| Natural behaviour and evolution continue | Breeding is managed, with a risk of inbreeding |
Zoos and the Central Zoo Authority
- The Central Zoo Authority was constituted in 1992 under the Wild Life (Protection) Act, 1972, after the amendment of 1991. No zoo in India may operate without its recognition, and it lays down standards of housing, upkeep and veterinary care and oversees conservation breeding.
- The National Zoo Policy of 1998 set out the role of Indian zoos as centres of conservation breeding, education and research rather than of display alone.
- Alipore Zoological Garden in Kolkata, opened in 1876, is the oldest formally established zoo in India.
- The Arignar Anna Zoological Park at Vandalur near Chennai is the largest zoo in the country by area; the Sri Chamarajendra Zoological Gardens at Mysuru, of 1892, are among the oldest and best known.
- The Padmaja Naidu Himalayan Zoological Park at Darjeeling is the highest altitude zoo in India and runs breeding programmes for the red panda and the snow leopard.
- The National Zoological Park in Delhi dates from 1959, and the Nehru Zoological Park in Hyderabad is known for its safari enclosures.
Botanical gardens
- The Acharya Jagadish Chandra Bose Indian Botanic Garden at Howrah, founded in 1787 by Robert Kyd, is the oldest and largest botanical garden in India and holds the famous Great Banyan Tree.
- The Botanical Survey of India, founded in 1890 with its headquarters at Kolkata, surveys the plant wealth of the country and maintains herbaria and gardens.
- The Lloyd Botanical Garden at Darjeeling conserves Himalayan flora, and the CSIR National Botanical Research Institute at Lucknow works on plant conservation and economic botany.
- The Botanic Garden of Indian Republic at Noida was set up as a national garden under the environment ministry.
Seed banks, gene banks and cryopreservation
- A seed bank stores dried seeds at low temperature; a gene bank stores seeds, pollen, tissue, semen, embryos or DNA.
- The National Gene Bank of the ICAR National Bureau of Plant Genetic Resources at New Delhi is one of the largest plant germplasm collections in the world.
- The parallel bureaux are the National Bureau of Animal Genetic Resources at Karnal, the National Bureau of Fish Genetic Resources at Lucknow and the National Bureau of Agriculturally Important Microorganisms at Mau.
- A national seed vault was built in the permafrost at Chang La in Ladakh, and the Svalbard Global Seed Vault in Norway acts as the world's backup collection of crop seeds.
- Cryopreservation keeps cells, tissue, pollen and embryos in liquid nitrogen at about minus 196 degrees Celsius, at which all metabolic activity stops.
- Orthodox seeds, such as those of wheat, rice and most pulses, can be dried and frozen and stored for decades. Recalcitrant seeds, such as those of mango, jackfruit, coconut and cocoa, lose viability when dried, so they are conserved as tissue cultures, field gene banks or cryobanks instead.
Captive breeding and reintroduction in India
- Conservation breeding centres for the Great Indian Bustard in the Jaisalmer region of Rajasthan, run with the Wildlife Institute of India, raise chicks from eggs collected in the wild for release into the Desert National Park.
- The cheetah, declared extinct in India in 1952, was reintroduced from Africa into Kuno National Park in Madhya Pradesh from 2022.
- The Crocodile Conservation Project of 1975 bred gharial, saltwater crocodile and mugger for release; the gharial centre at Kukrail near Lucknow is the best known.
- Vulture conservation breeding centres, the first at Pinjore in Haryana, breed white-backed, long-billed and slender-billed vultures whose numbers crashed after the veterinary use of diclofenac.
- The pygmy hog programme in Assam breeds and releases the world's smallest wild pig into the grasslands of Manas and nearby reserves.
Why ex-situ conservation is only a second line
- Only a few individuals can be kept, so the gene pool is small and inbreeding depression follows.
- Animals bred in captivity may lose the ability to hunt, avoid predators or migrate, which lowers survival after release.
- It is expensive per animal and cannot preserve the ecological relationships of the habitat.
- Recalcitrant seeds and many micro-organisms still cannot be stored reliably.
- So ex-situ work is a support for in-situ conservation and a safeguard against extinction, not a substitute for protecting habitat.
Exam Point of View
Papers test the in-situ and ex-situ distinction first: a list is given and the candidate must pick the ex-situ item, where a biosphere reserve or a sanctuary is slipped in as the distractor. Then come the fixed facts: 1992 and the Wild Life (Protection) Act for the Central Zoo Authority, 1876 and Kolkata for the oldest zoo, 1787 and Robert Kyd for the Howrah garden, 1890 and Kolkata for the Botanical Survey of India, minus 196 degrees Celsius for cryopreservation, and the orthodox and recalcitrant seed pair. Programme and place matching is also common: bustard with Rajasthan, cheetah with Kuno, gharial with Kukrail, vulture with Pinjore, pygmy hog with Assam, red panda with Darjeeling.
Important Facts
| Central Zoo Authority | Constituted 1992 under the Wild Life (Protection) Act, 1972 |
|---|---|
| National Zoo Policy | 1998 |
| Oldest zoo in India | Alipore Zoological Garden, Kolkata, 1876 |
| Largest zoo by area | Arignar Anna Zoological Park, Vandalur, Chennai |
| Highest altitude zoo | Padmaja Naidu Himalayan Zoological Park, Darjeeling |
| Oldest botanical garden | Indian Botanic Garden, Howrah, 1787, founded by Robert Kyd |
| Botanical Survey of India | Founded 1890, headquarters Kolkata |
| National Gene Bank | ICAR National Bureau of Plant Genetic Resources, New Delhi |
| Animal and fish gene banks | NBAGR at Karnal and NBFGR at Lucknow |
| Cryopreservation temperature | About minus 196 degrees Celsius in liquid nitrogen |
| Recalcitrant seeds | Mango, jackfruit, coconut and cocoa; cannot be dried and frozen |
| Cheetah reintroduction site | Kuno National Park, Madhya Pradesh, from 2022 |
| Vulture breeding centre | Pinjore, Haryana |
Practice MCQs on this topic
Which of the following is an example of ex-situ conservation?
- A.Biosphere reserve
- B.Wildlife sanctuary
- C.National park
- D.Botanical garden
Show answer
Explanation
The correct answer is D, a botanical garden. Ex-situ conservation means keeping a species, or only its germplasm, outside its natural habitat, which is what a botanical garden, a zoo, an aquarium, a seed bank, a gene bank or a conservation breeding centre does. Option A is wrong because a biosphere reserve conserves a whole landscape with its core, buffer and transition zones, which is in-situ conservation. Option B is wrong because a wildlife sanctuary protects animals where they live, with limited human activity allowed, again in-situ. Option C is wrong because a national park is the strictest form of in-situ protection, where grazing and private rights are not permitted. The quickest test is to ask whether the species stays in its habitat; if it does, the method is in-situ.
The Central Zoo Authority was constituted under which law?
- A.Wild Life (Protection) Act, 1972
- B.Environment (Protection) Act, 1986
- C.Biological Diversity Act, 2002
- D.Forest (Conservation) Act, 1980
Show answer
Explanation
The correct answer is A, the Wild Life (Protection) Act, 1972. The amendment of 1991 inserted the provisions on zoos into that Act, and the Central Zoo Authority was constituted in 1992 to recognise zoos, prescribe standards for animal housing, upkeep and veterinary care and coordinate conservation breeding; a zoo without its recognition cannot operate. Option B is wrong because the Environment (Protection) Act of 1986 is the umbrella law under which notifications such as eco-sensitive zones and coastal regulation zones are issued. Option C is wrong because the Biological Diversity Act of 2002 created the National Biodiversity Authority, which deals with access to biological resources and benefit sharing. Option D is wrong because the Forest (Conservation) Act of 1980 governs the diversion of forest land for non-forest use.
Which is the oldest formally established zoo in India, opened in 1876?
- A.National Zoological Park, Delhi
- B.Alipore Zoological Garden, Kolkata
- C.Nehru Zoological Park, Hyderabad
- D.Arignar Anna Zoological Park, Chennai
Show answer
Explanation
The correct answer is B, the Alipore Zoological Garden in Kolkata, opened to the public in 1876 and therefore the oldest formally established zoo in the country. Option A is wrong because the National Zoological Park in Delhi dates from 1959 and was planned as a model zoo for the capital. Option C is wrong because the Nehru Zoological Park at Hyderabad was opened in the 1960s and is known for its safari enclosures and nocturnal animal house. Option D is wrong because the Arignar Anna Zoological Park at Vandalur near Chennai, though the largest zoo in India by area, was opened in 1985 after shifting from the older Madras zoo. A related fact often asked alongside is that the Mysuru zoo of 1892 is the second of the old zoos and the Darjeeling zoo the highest in altitude.
The Indian Botanic Garden at Howrah was founded in 1787 by:
- A.William Roxburgh
- B.Robert Kyd
- C.Joseph Hooker
- D.Jagadish Chandra Bose
Show answer
Explanation
The correct answer is B, Robert Kyd, an officer of the East India Company who proposed and founded the garden at Shibpur in Howrah in 1787, first for growing plants of economic value such as teak and spices. Option A is wrong because William Roxburgh, often called the father of Indian botany, was its superintendent after Kyd and compiled the Flora Indica, which is a different contribution. Option C is wrong because Joseph Hooker travelled in the Himalaya and the Khasi hills and wrote on their flora but did not found this garden. Option D is wrong because the garden was renamed the Acharya Jagadish Chandra Bose Indian Botanic Garden in his honour in 2009, long after his death, which is why his name is attached to it today. The garden holds the Great Banyan Tree.
The National Gene Bank, one of the largest plant germplasm collections in the world, is maintained by which institution?
- A.National Bureau of Plant Genetic Resources, New Delhi
- B.Botanical Survey of India, Kolkata
- C.National Botanical Research Institute, Lucknow
- D.Indian Agricultural Research Institute, Pusa
Show answer
Explanation
The correct answer is A, the National Bureau of Plant Genetic Resources at New Delhi, an institute of the Indian Council of Agricultural Research, which holds the National Gene Bank where seeds of crop plants and their wild relatives are stored at low temperature for long-term conservation. Option B is wrong because the Botanical Survey of India, founded in 1890 with its headquarters at Kolkata, surveys and documents plant wealth and maintains herbaria and gardens rather than a crop gene bank. Option C is wrong because the National Botanical Research Institute at Lucknow, a CSIR laboratory, works on plant conservation, taxonomy and economic botany. Option D is wrong because the Indian Agricultural Research Institute at Pusa in Delhi is a research and teaching institute; the gene bank stands on the same campus but belongs to the Bureau.
The headquarters of the Botanical Survey of India, founded in 1890, is located at:
- A.Dehradun
- B.Kolkata
- C.Lucknow
- D.New Delhi
Show answer
Explanation
The correct answer is B, Kolkata. The Botanical Survey of India was founded in 1890 with its headquarters in Kolkata, close to the Indian Botanic Garden at Howrah, and it surveys the plant resources of the country, publishes floras and maintains herbaria and botanical gardens through its regional circles. Option A is wrong because Dehradun is the headquarters of the Forest Survey of India, the Wildlife Institute of India and the Forest Research Institute, and this is the likeliest confusion in the question. Option C is wrong because Lucknow houses the National Botanical Research Institute and the National Bureau of Fish Genetic Resources. Option D is wrong because New Delhi has the National Bureau of Plant Genetic Resources and the environment ministry, not the Survey's headquarters. Pair 1890 and Kolkata together.
Seeds that cannot be dried and frozen for long-term storage in a seed bank are called:
- A.Orthodox seeds
- B.Recalcitrant seeds
- C.Dormant seeds
- D.Hybrid seeds
Show answer
Explanation
The correct answer is B, recalcitrant seeds. These seeds lose their viability when their moisture content is reduced, so they cannot be kept in the cold rooms of a seed bank; mango, jackfruit, coconut, cocoa and most tropical fruit trees are examples, and they are conserved instead in field gene banks, as tissue cultures or in cryobanks. Option A is wrong because orthodox seeds, such as wheat, rice, maize and most pulses, can be dried to a low moisture content and frozen, and they are the seeds that gene banks store for decades. Option C is wrong because dormancy is a resting state from which a seed can germinate when conditions are right, and it says nothing about storage. Option D is wrong because hybrid refers to parentage, not to storage behaviour.
Cryopreservation of pollen, tissue and embryos is carried out in liquid nitrogen at a temperature of about:
- A.Minus 20 degrees Celsius
- B.Minus 80 degrees Celsius
- C.Minus 196 degrees Celsius
- D.Minus 273 degrees Celsius
Show answer
Explanation
The correct answer is C, about minus 196 degrees Celsius, the boiling point of liquid nitrogen, at which all metabolic activity in the stored material stops and the germplasm can be kept for very long periods without change. Option A is wrong because minus 20 degrees is the temperature of ordinary long-term seed storage in a gene bank, not of cryopreservation. Option B is wrong because minus 80 degrees is the range of deep freezers used for vaccines and biological samples. Option D is wrong because minus 273 degrees Celsius is close to absolute zero, which laboratory cryopreservation does not use. Remember the pair: minus 20 degrees for seed banks and minus 196 degrees for cryobanks, because examiners place the two figures in the same set of options.
The conservation breeding centres for the Great Indian Bustard are located in which state?
- A.Gujarat
- B.Rajasthan
- C.Maharashtra
- D.Madhya Pradesh
Show answer
Explanation
The correct answer is B, Rajasthan. The breeding centres stand in the Jaisalmer region, near the Desert National Park, which holds the largest surviving population of the bird, and they are run by the state forest department with the Wildlife Institute of India: eggs are collected from the wild, hatched in incubators and the chicks reared for release. Option A is wrong, though Gujarat has bustard habitat in the Kutch grasslands and is part of the recovery programme. Option C is wrong because Maharashtra has a small population in the Nannaj area of Solapur but no breeding centre of this kind. Option D is wrong because Madhya Pradesh is associated with the cheetah reintroduction at Kuno, not with the bustard. The Great Indian Bustard is the state bird of Rajasthan, which makes the pairing easier to remember.
The cheetah, which had become extinct in India, was reintroduced into which national park?
- A.Kanha National Park
- B.Kuno National Park
- C.Bandhavgarh National Park
- D.Panna National Park
Show answer
Explanation
The correct answer is B, Kuno National Park in the Sheopur area of Madhya Pradesh, where cheetahs brought from Namibia and South Africa were released from 2022 under the cheetah reintroduction project; the cheetah had been declared extinct in India in 1952. Option A is wrong because Kanha, in the Maikal range, is a tiger reserve best known for saving the hard-ground barasingha. Option C is wrong because Bandhavgarh is a tiger reserve famous for its fort and its high density of tigers. Option D is wrong because Panna is remembered for the successful reintroduction of tigers after the local population was wiped out, which is a different programme often confused with the cheetah project. Kuno had earlier been prepared as a second home for the Asiatic lion of Gir.
Which zoo in India, the highest in altitude, is known for breeding the red panda and the snow leopard?
- A.Padmaja Naidu Himalayan Zoological Park, Darjeeling
- B.Sri Chamarajendra Zoological Gardens, Mysuru
- C.Alipore Zoological Garden, Kolkata
- D.Nandankanan Zoological Park, Bhubaneswar
Show answer
Explanation
The correct answer is A, the Padmaja Naidu Himalayan Zoological Park at Darjeeling, the highest altitude zoo in India, which runs recognised conservation breeding programmes for the red panda, the snow leopard and the Himalayan wolf, and has released captive-bred red pandas into the Singalila landscape. Option B is wrong because the Mysuru zoo of 1892, though among the oldest and most visited, is not a high-altitude zoo and is known for its wide range of exotic species. Option C is wrong because the Alipore zoo in Kolkata is the oldest in the country, dating from 1876. Option D is wrong because Nandankanan near Bhubaneswar is famous for white tigers and for being the first zoo in India to breed the gharial in captivity, which is itself a frequently asked fact.
Frequently Asked Questions
What is the difference between in-situ and ex-situ conservation?
In-situ conservation protects a species in its natural habitat, so the whole ecosystem is conserved, as in national parks, sanctuaries and biosphere reserves. Ex-situ conservation keeps the species or only its germplasm outside the habitat, in zoos, botanical gardens, seed and gene banks, aquaria and breeding centres.
Under which law was the Central Zoo Authority set up?
Under the Wild Life (Protection) Act, 1972, as amended in 1991; the Authority was constituted in 1992. It recognises zoos, lays down standards for animal housing and veterinary care, and coordinates conservation breeding, and no zoo may operate without its recognition.
Which is the oldest botanical garden in India?
The Acharya Jagadish Chandra Bose Indian Botanic Garden at Howrah near Kolkata, founded in 1787 by Colonel Robert Kyd of the East India Company. It is also the largest in the country and is famous for the Great Banyan Tree, whose canopy covers more than a hectare.
What are recalcitrant seeds?
Seeds that cannot survive drying and freezing, so they cannot be kept in an ordinary seed bank. Mango, jackfruit, coconut and cocoa are examples. Such species are conserved in field gene banks, as tissue cultures or in cryobanks instead of as stored seed.
Why is ex-situ conservation not enough by itself?
Only a small number of individuals can be kept, so inbreeding sets in; captive animals may lose the skills needed to survive in the wild; the cost per animal is high; and the habitat and its web of relationships are not conserved at all. It works as a safeguard alongside in-situ protection.
Sources
- Zoos of India and Recognition of Zoos — Central Zoo Authority, Ministry of Environment, Forest and Climate Change
- Biology (Class XII), Chapter 15: Biodiversity and Conservation — NCERT
- National Gene Bank and Plant Germplasm Conservation — ICAR-National Bureau of Plant Genetic Resources





