A leaf was plucked from a plant on a sunny day and kept for 2 minutes in boiling water. It was subsequently immersed in boiling alcohol and treated with iodine solution. What will be the final colour of the leaf after the test?
- A.Colourless
- B.Green
- C.Blue
- D.White
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Explanation
The correct answer is C, Blue. A leaf from a sunny day is full of starch made by photosynthesis, and iodine turns starch blue-black.
This is the standard school test for starch in a leaf. Boiling the leaf in water kills its cells and softens it so that the solutions can enter. Boiling it in alcohol, usually over a water bath because alcohol catches fire easily, dissolves the chlorophyll, leaving the leaf pale or nearly colourless. When iodine solution is then added, the starch stored in the leaf reacts with it and the leaf turns blue-black. A leaf kept in the dark for two days before the test shows no blue colour, proving that light is needed for photosynthesis.
A is wrong: the leaf is colourless only before iodine is added; the starch then turns it blue.
B is wrong: the green chlorophyll has already been removed by the boiling alcohol.
D is wrong: a starch-free leaf would show the brown colour of iodine, not white, and this leaf has starch.
Exam tip: iodine test: starch gives blue-black; alcohol removes chlorophyll before the test.