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Railway GK Questions 2026

Static GK in railway exams starts with one shared block: Class 10 science. Every RRB exam on GK24 tests it. Group D has 25 questions on Physics, Chemistry and Life Sciences of 10th CBSE level. JE has 30 in CBT-1 and another 15 on Physics and Chemistry in CBT-2. ALP tests General Science in CBT-1 and Basic Science and Engineering in CBT-2, and NTPC lists general science up to 10th inside General Awareness. Beyond science, the lists differ. NTPC names history and the freedom struggle, geography, polity, the economy, art and culture, environment, computers, abbreviations, transport systems in India, world organisations and government bodies. JE names geography, history and culture, polity, the economy and environmental issues, and adds computers and pollution control in CBT-2. This page holds GK24's topic-wise GK notes for railway exams in English and Hindi: General Science, History, Geography, Polity, Economy, Art and Culture, Environment, Computers and static GK, including Indian Railways. Each note is short facts and tables that can be revised in one sitting. Begin with the science notes, since they serve every railway exam, then add the static topics your exam lists, and test each note with its quiz.

Railway GK Questions with Answers

Q6841.Uttar PradeshMedium

Which river is the longest tributary of the Ganga?

  1. A.Ghaghara
  2. B.Yamuna
  3. C.Gomti
  4. D.Son
Show answer
Correct answer: B. Yamuna

The Yamuna rises at the Yamunotri glacier in Uttarakhand, flows past Delhi, Mathura and Agra and joins the Ganga at Prayagraj after a course of more than thirteen hundred kilometres, which makes it the longest tributary of the Ganga.

Q6851.Science & TechnologyHard

The Genome India Project, launched in January 2020 to build a reference database of Indian genomes, is coordinated by

  1. A.The Indian Council of Medical Research
  2. B.The Department of Biotechnology
  3. C.The Indian Space Research Organisation
  4. D.The Central Drug Standard Control Organisation
Show answer
Correct answer: B. The Department of Biotechnology

The Genome India Project was launched in January 2020 by the Department of Biotechnology, which works under the Ministry of Science and Technology, and brings together about twenty academic institutions to sequence Indian genomes and build a reference set that reflects the country's genetic diversity.

Q6852.Science & TechnologyHard

The Centre for DNA Fingerprinting and Diagnostics, set up in 1995 under the Department of Biotechnology, is located at

  1. A.Pune
  2. B.Hyderabad
  3. C.Kalyani
  4. D.Lucknow
Show answer
Correct answer: B. Hyderabad

The Centre for DNA Fingerprinting and Diagnostics was established in 1995 as an autonomous institution of the Department of Biotechnology and works at Hyderabad on DNA fingerprinting services, diagnostics of genetic disorders and research in genomics.

Q6854.Science & TechnologyMedium

Which enzyme is used in the polymerase chain reaction because it remains active at high temperature?

  1. A.DNA ligase
  2. B.Taq polymerase
  3. C.Restriction endonuclease EcoRI
  4. D.Reverse transcriptase
Show answer
Correct answer: B. Taq polymerase

The polymerase chain reaction, invented by Kary Mullis in 1983, repeats three steps: the DNA is denatured near ninety-five degrees Celsius, short primers anneal at a lower temperature, and the strand is extended at about seventy-two degrees.

Q6856.Science & TechnologyMedium

During gel electrophoresis, DNA fragments move towards which electrode, and why?

  1. A.Towards the cathode, because DNA is positively charged
  2. B.Towards the anode, because DNA is negatively charged
  3. C.Towards the cathode, because DNA is neutral but light
  4. D.They do not move; only the dye moves
Show answer
Correct answer: B. Towards the anode, because DNA is negatively charged

Each nucleotide of DNA carries a phosphate group, and the phosphate backbone gives the whole molecule a net negative charge at the pH used in the laboratory.

Q6857.Science & TechnologyMedium

In the CRISPR-Cas9 system, the Cas9 protein acts as

  1. A.A ligase that joins DNA fragments
  2. B.An endonuclease that cuts both strands of DNA
  3. C.A polymerase that copies DNA
  4. D.A plasmid that carries a gene into the cell
Show answer
Correct answer: B. An endonuclease that cuts both strands of DNA

Cas9 is an enzyme that breaks the sugar-phosphate backbone of both DNA strands at the point where its guide RNA has bound, provided a short adjacent signal called the PAM is present.

Q6858.Science & TechnologyMedium

The Nobel Prize in Chemistry 2020 was awarded for the development of the CRISPR-Cas9 genome editing method to

  1. A.James Watson and Francis Crick
  2. B.Emmanuelle Charpentier and Jennifer Doudna
  3. C.Kary Mullis and Michael Smith
  4. D.Werner Arber and Daniel Nathans
Show answer
Correct answer: B. Emmanuelle Charpentier and Jennifer Doudna

The two scientists showed that the bacterial CRISPR-Cas9 system could be directed by a designed guide RNA to cut any chosen sequence of DNA, which turned a microbial defence mechanism into a general tool for editing genomes, and they shared the Nobel Prize in Chemistry in 2020.

Q6859.Science & TechnologyEasy

DNA fingerprinting was developed in 1984 by which scientist?

  1. A.Kary Mullis
  2. B.Alec Jeffreys
  3. C.Frederick Sanger
  4. D.Hamilton Smith
Show answer
Correct answer: B. Alec Jeffreys

Working at the University of Leicester in 1984, Alec Jeffreys found that certain short sequences, the minisatellites or variable number tandem repeats, repeat a different number of times in different people, and that a pattern made from them identifies an individual.

GK Questions for sub-exams