Cryopreservation of pollen, tissue and embryos is carried out in liquid nitrogen at a temperature of about:
- A.Minus 20 degrees Celsius
- B.Minus 80 degrees Celsius
- C.Minus 196 degrees Celsius
- D.Minus 273 degrees Celsius
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The correct answer is C, about minus 196 degrees Celsius, the boiling point of liquid nitrogen, at which all metabolic activity in the stored material stops and the germplasm can be kept for very long periods without change.
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