Which enzyme is used in the polymerase chain reaction because it remains active at high temperature?
- A.DNA ligase
- B.Taq polymerase
- C.Restriction endonuclease EcoRI
- D.Reverse transcriptase
Show answer
The polymerase chain reaction, invented by Kary Mullis in 1983, repeats three steps: the DNA is denatured near ninety-five degrees Celsius, short primers anneal at a lower temperature, and the strand is extended at about seventy-two degrees.
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