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General ScienceMedium

Which body gives approval for the release of genetically modified organisms in India?

  1. A.Indian Council of Agricultural Research
  2. B.Genetic Engineering Appraisal Committee
  3. C.Food Safety and Standards Authority of India
  4. D.Central Pollution Control Board

Correct answer

B. Genetic Engineering Appraisal Committee

Explanation

The correct answer is B, the Genetic Engineering Appraisal Committee. This committee works under the Ministry of Environment, Forest and Climate Change and appraises proposals for the large scale use and the environmental release of genetically engineered organisms and products, so no transgenic crop may be grown commercially without its clearance. Option A, the Indian Council of Agricultural Research, conducts agricultural research and runs the network of institutes and universities, but it is not the regulator for release. Option C, the Food Safety and Standards Authority of India, sets standards for food and so has a say in labelling and safety, not in environmental release. Option D, the Central Pollution Control Board, deals with air and water pollution under its own Acts. The Department of Biotechnology, created in 1986, promotes the subject rather than regulating release.

Read the full article: Biotechnology and Its Applications: Notes and PYQs

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Q1.General ScienceEasy

Which enzyme cuts DNA at a specific recognition sequence and is therefore called molecular scissors?

  1. A.DNA ligase
  2. B.Restriction endonuclease
  3. C.DNA polymerase
  4. D.Reverse transcriptase
Show answer
Correct answer: B. Restriction endonuclease

A restriction endonuclease recognises a short palindromic sequence in DNA and cuts both strands there, leaving sticky ends that can pair with another piece cut by the same enzyme; Hind II was the first such enzyme to be discovered and EcoRI from Escherichia coli is the most widely used.

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Q2.General ScienceMedium

Which bacterium is used as a natural vector to transfer genes into plant cells?

  1. A.Escherichia coli
  2. B.Agrobacterium tumefaciens
  3. C.Bacillus thuringiensis
  4. D.Rhizobium leguminosarum
Show answer
Correct answer: B. Agrobacterium tumefaciens

This soil bacterium naturally transfers a part of its tumour inducing plasmid, called the Ti plasmid, into the cells of a dicotyledonous plant and causes crown gall disease; genetic engineers disarm the plasmid, insert the gene they want and use the bacterium as a vector, which is why it is…

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Q5.General ScienceMedium

Which heat stable enzyme is used in the polymerase chain reaction?

  1. A.Taq polymerase
  2. B.DNA ligase
  3. C.EcoRI
  4. D.Lysozyme
Show answer
Correct answer: A. Taq polymerase

The polymerase chain reaction repeats three steps, denaturation at a high temperature, annealing of short primers and extension of the new strand, so the enzyme must survive near boiling water; Taq polymerase, obtained from the bacterium Thermus aquaticus of hot springs, does exactly that and lets a single DNA segment…

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